Effect of Warm Acupuncture on “Dinghui Acupoint” and “Heart Acupoint” in Traditional Mongolian Medicine on Behaviors and Hypothalamic Inammatory Cytokines in Rats with Chronic Fatigue Syndrome

Shumei Bai (Inner Mongolia Medical University Hulunbuir Municipal Mongolian Medical Hospital)
Ling Shui (Inner Mongolia Medical University)
Qin Si (Inner Mongolia Medical University)
Yingsong Chen (Inner Mongolia Medical University)

Abstract


Objective: To observe the effects of warm acupuncture on “Dinghui Acupoint” and “Heart Acupoint” in Traditional Mongolian Medicine on behavior and hypothalamic inflammatory cytokines IL-1β, IL-6 and IFN-r in Rats with Chronic Fatigue Syndrome. Methods: SD rats were randomly divided into normal group, model group, warm acupuncture group and moxibustion positive control group. The latter three groups of rats were used to establish a model of rats with chronic fatigue syndrome (CFS) using a combination of physical fatigue and mental fatigue. When establishing the model of warm acupuncture group, “Dinghui Acupoint” and “Heart Acupoint” intervention was carried out; when establishing the model of moxibustion positive control group, “Zusanli Acupoint” intervention was carried out on both sides. Behavioral observations (body weight, exhaustive swimming time, tail suspension experiment, water maze) were performed before and after modeling. The hypothalamic inflammatory cytokines IL-1β, IL-6 and IFN-r were detected by ELISA method after warm acupuncture and moxibustion intervention. Results: After 21 days of modeling, the body weight of the rats in each group was significantly lower than that in the normal group, and there was a significant difference (P<0.01); Compared with the model group, the weight of the rats in the warm acupuncture group increased significantly, and there was a significant difference (P<0.01); Compared with the model group, the exhaustive swimming time of the rats in the warm acupuncture group was significantly prolonged, and there was a significant difference (P<0.01); Compared with the moxibustion group, the exhaustion time of the rats in the warm acupuncture group was relatively prolonged, and there was a significant difference (P<0.05); Compared with the normal group, the tail suspension time of the model group was significantly prolonged, and there was a significant difference (P<0.05); Compared with the model group, there was a significant difference in the duration of the suspension of the warm acupuncture group and the moxibustion group (P<0.01); Compared with the normal group, the total distance of the water maze test was shorter in the model group, and there was a significant difference (P<0.01); Compared with the model group, both the warm acupuncture group and the moxibustion group were prolonged, and there was a significant difference (P<0.05); Compared with the moxibustion group, the distance between the rats in the warm acupuncture group was relatively longer, but there was no significant difference (P>0.05); Compared with the normal group, IL-1β, IL-6 and IFN-r increased significantly in the model group and there was a significant difference (P<0.05); Compared with the model group, IL-1β and IL-6 in the warm acupuncture group was significantly decreased (P<0.05), and the IL-6 in the moxibustion group was significantly different (P<0.05); Compared with the model group, there was no significant difference between the IFN-r group and the moxibustion group (P>0.05); Compared with the moxibustion group, the levels of IL-1β, IL-6 and IFN-r were not significantly different (P>0.05). Conclusion: Warm acupuncture on “Dinghui Acupoint” and “Heart Acupoint” in Traditional Mongolian Medicine has the ability to improve the body’s defense and self-healing ability, improve chronic fatigue syndrome (CFS), and thus play a preventive role. The results of this research indicate that the warm acupuncture group and the moxibustion group have the same effect.


Keywords


Warm acupuncture in Traditional Mongolian Medicine; Chronic fatigue syndrome (CFS); Behaviors; Cytokines

Full Text:

PDF

References


Xinghua Chen, Luxi Li, Wen Zhang et al. Randomized controlled study of acupuncture on chronic fatigue syndrome[J]. Chinese Acupuncture, 2010, 30(7): 533-536. (in Chinese)

Xingzhong Feng, Min Jiang, Wei Lu et al. Clinical study of Shushun Granule in the treatment of chronic fatigue syndrome[J]. Chinese Journal of Traditional Chinese Medicine, 2010, 25(11):1877-1879. (in Chinese)

PALL ML. Elevated, sustained peroxynitrite levels as the cause of chronic fatigue syndrome [J]. Medical Hypotheses, 2000, 54(1): 115-125.

,[12] Yingsong Chen, Agura. Mongolian doctor warm acupuncture therapy for the treatment of chronic fatigue syndrome[J]. Journal of Liaoning University of Traditional Chinese Medicine, 2006, 8(5):116-117. (in Chinese)

Saiyin Chao Ketu, Su. Chaolumen, Bo. Agulha. Psychological analysis of patients with chronic fatigue syndrome and intervention of Mongolian doctor warm acupuncture[J]. Chinese Journal of Traditional Medicine, 2012(6):36-38. (in Chinese)

Chuanwei Chen. Clinical and functional mechanism of acupuncture intervention on chronic fatigue syndrome[D]. Guangzhou: Guangzhou University of Traditional Chinese Medicine, 2010:24. (in Chinese)

Qizhao Yang. Study on the mechanism of intervention of the back-deep line embedding in rats with chronic fatigue syndrome[D]. Guangzhou: Guangzhou University of Traditional Chinese Medicine, 2015:33-34. (in Chinese)

Yinghua Luo, Chunzhi Tang, Weijun Luo.Effects of moxibustion on back inflammatory stress on chronic fatigue rats[J]. Journal of New Chinese Medicine, 2012, 88(8):161-162. (in Chinese)

Maes M, Twisk FN, Kubera M, et al. Evidence for inflammation and activation of cell-mediated immunity in Myalgic Encephalomyelitis/chronic futigue syndrome: increased interleukin-1,tumar necrosis factor-a,PME-elastase,lysozyme and neopterin. J Affect Disord, 2012, 136(3): 933-939.

Neurath MF, Finotto S. IL-6 signaling in autoimmunity, chronic imfammation and imfammation-associated cancer. Cytokine Growth Factor Rew, 2011, 22(2): 83-89.

Dinarello CA. Immunological and inflammatory functions of the IL-1family. Annu Rcv Immunol, 2009, 27: 519-550.

Lan Wu, Hasi Yao, Gerier et al. Therapeutic effect of Mongolian doctor warm acupuncture on lumbar disc herniation[J]. Chinese Journal of Traditional Medicine, 2015, 4(4):1-3. (in Chinese)

Wen Jin, Xiaoyong Li et al. Study on the establishment of chronic fatigue rat model[J].Journal of Shandong University of Traditional Chinese Medicine, 2008, 32(2):107. (in Chinese)

Xiaojuan Hong, Xi Wu, Yan. Preparation and detection of animal model of chronic fatigue syndrome[J].Liaoning Journal of Traditional Chinese Medicine, 2011, 38(4):737-738. (in Chinese)

Yumin Lin. Immunological intervention and clinical study of moxibustion on chronic fatigue model rats[D]. Guangzhou: Guangzhou University of Traditional Chinese Medicine, 2014, 9. (in Chinese)

Xingmin Li, Mingxu Wang. Modern Behavioral Medicine[M]. Beijing: 2013.1. (in Chinese)

Ximing Yu, Siyuan Chen, Wangyi Yu. Establishment and evaluation of animal model of chronic fatigue syndrome in traditional Chinese medicine[J].Basic Medical Sciences, 2014, 4(2):23-24. (in Chinese)

Huo Bren. A preliminary study on the mechanism of warming acupuncture of Mongolian medicine[J]. Chinese Journal of Traditional Medicine, 2006, 9(5):76-77. (in Chinese)

Yingsong Chen, Agulha, Su. Chaolumen et al. Anti-inflammatory analgesic and immune effects of Mongolian doctor warm acupuncture[J]. Neurology and Mental Health, 2010, 10(6):594. (in Chinese)



DOI: https://doi.org/10.30564/jams.v2i2.456

Refbacks

  • There are currently no refbacks.
Copyright © 2019 shumei bai Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.