Callus Cultures Of Beans Infected With Virus As A Model For Testing Antiviral Compaunds

Kovalenko Oleksiy (Zobolotny Institute of Microbiology and Virology NAS of Ukraine, Kyiv, 03143, Ukraine)
Kyrychenko Angelina (Zobolotny Institute of Microbiology and Virology NAS of Ukraine, Kyiv, 03143, Ukraine)
Kovalenko Olena (Zobolotny Institute of Microbiology and Virology NAS of Ukraine, Kyiv, 03143, Ukraine)


In the work, bean callus raised from a leaves of Bean common mosaic virus infected bean plant was obtained and adapted for the testing of antiviral activity of liposomal glycan-glycolipid complexes. Ganoderma adspersum glucans and Pseudomonas spec. rhamnolipids were constituents of liposomal compaunds. It has been shown that under the long-term cultivation (up to 3 months) in the presence of a liposomal preparation containing (10-100 mg/l), the virus is eliminated from the tissue. This is evidenced by the absence of 391 bp sequence amplification product established by RT-PCR in the callus tissue, cultured on a medium containing the liposomal complex. The proposed model system is analogous to plant tumors and has obvious advantages over similar systems in vivo, since the callus growth is controlled and independent of environmental factors.


Bean common mosaic virus (BCMV); Bean yellow mosaic virus (BYMV); Reverse transcriptase polymerase chain reaction (RT-PCR); Plant tumors; Glycans; Glycolipids; Therapy of virus diseases

Full Text:



J. Gohlke, R. Deeken, Plant responses to Agrobacterium tumefaciens and crown gall development, [J]. Front Plant Sci., 2014, 5: 155.

M. Ikeuchi, K. Sugimoto, A. Iwase. Plant callus: mechanisms of induction and repression, [J]. Plant Cell., 2013, 25(9): 3159 – 3173.

I.S. Shcherbatenko, L.T. Oleshchenko, Somaclonal variation as a sourse of tomato spotted wilt virus-resistance in plants, [C]. Virus diseases and crop biosecurity, Dordrecht: Springer, 2006: 113-143.

A.N. Kyrychenko, A.G. Kovalenko, Bean common mosaic in the Kiev region: etiology disease and pathoden identivication, [J]. Microbial J., 2018, 80: 96 – 107. (In Ukrainian)

A.M. Kyrychenko, I.O. Antipov, K.V. Hrynchuk. Phylogenetic analysis of Ukrainian BYMV isolates from soybeans and beans, [J]. Cytol Genet., 2017, 51(3): 173 – 178. (In Ukrainian)

O.G. Kovalenko, V.M. Vasilev, N.I. Adamchuk-Chala, L.V Tytova, E.V. Karpenko, Artifical glycan-glycolipid complexes as antiviral means and effectors of microbial preparations on the base of rhizobia, [J]. Dopov. Nac. acad. nauk Ukr, 2017, 1: 88 – 96 (In Ukrainian)

O.G. Kovalenko, S.P. Wasser, Glycans of higher Basidiomycetes mushrooms with antiphytoviral properties: isolation, characterization, and biological activity. Fungi and their Applications in the series of Progress in Mycological Research. Eds: Deshmukh S.K., Mishra J.K., Tewari, Japla P., Papp T. Publisher: CRC Press/Taylor & Francis Group, LLC, 2016: 161-200.

I. Antipov, K. Hrynchuk, О. Duplyak, Development PCR systems for identification of mosaic virus ordinary beans (Bean common mosaic virus). [J]. Scientific herald of NULES of Ukraine. Series: biology, biotechnology, ecology, 2016, 234: 40-46. (In Ukrainian).

I.O. Antipov, PCR diagnostic of the bean yellow mosaic virus, [J]. Sci. Proc. Nat. Univ. Life and Pryrodokorystuavnnya Ukraine, 2014, 204: 151 – 154.

P.N. Sharma, V. Sharma, A. Sharma, K. Raiput, S.K. Sharma, Identification and molecular characterization of Bean yellow mosaic virus infecting French bean in Himachal Pradesh, [J]. Virus disease, 2015, 26: 315 – 318.

M.C. Polanco, M.L. Ruiz, Effect of benzylaminopurine on in vitro and in vivo root development in lentil (Lens culinaris Medik.), [J]. Plant Cell Rep., 1997, 17: 22 – 26.



  • There are currently no refbacks.
Copyright © 2019 Kovalenko Oleksiy, Kyrychenko Angelina, Kovalenko Olena Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.