Callus Cultures Of Beans Infected With Virus As A Model For Testing Antiviral Compaunds

Kovalenko Oleksiy(Zobolotny Institute of Microbiology and Virology NAS of Ukraine, Kyiv, 03143, Ukraine)
Kyrychenko Angelina(Zobolotny Institute of Microbiology and Virology NAS of Ukraine, Kyiv, 03143, Ukraine)
Kovalenko Olena(Zobolotny Institute of Microbiology and Virology NAS of Ukraine, Kyiv, 03143, Ukraine)

Abstract


In the work, bean callus raised from a leaves of Bean common mosaic virus infected bean plant was obtained and adapted for the testing of antiviral activity of liposomal glycan-glycolipid complexes. Ganoderma adspersum glucans and Pseudomonas spec. rhamnolipids were constituents of liposomal compaunds. It has been shown that under the long-term cultivation (up to 3 months) in the presence of a liposomal preparation containing (10-100 mg/l), the virus is eliminated from the tissue. This is evidenced by the absence of 391 bp sequence amplification product established by RT-PCR in the callus tissue, cultured on a medium containing the liposomal complex. The proposed model system is analogous to plant tumors and has obvious advantages over similar systems in vivo, since the callus growth is controlled and independent of environmental factors.


Keywords


Bean common mosaic virus (BCMV); Bean yellow mosaic virus (BYMV); Reverse transcriptase polymerase chain reaction (RT-PCR); Plant tumors; Glycans; Glycolipids; Therapy of virus diseases

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DOI: https://doi.org/10.30564/jrb.v1i2.1252

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