Genetic Evaluation of Starch Synthesis-Related Genes and Starch Quality Traits in Special Rice Resources

Linyu Tai (Chongqing Normal University, Chongqing Engineering Research Center of Specialty Crop Resource, Chongqing,401331, China)
Lixia Lei (Chongqing Normal University, Chongqing Engineering Research Center of Specialty Crop Resource, Chongqing,401331, China)
Lan Luo (Chongqing Normal University, Chongqing Engineering Research Center of Specialty Crop Resource, Chongqing,401331, China)
Hang Shen (Chongqing Normal University, Chongqing Engineering Research Center of Specialty Crop Resource, Chongqing,401331, China)
Jiao Chen (Chongqing Normal University, Chongqing Engineering Research Center of Specialty Crop Resource, Chongqing,401331, China)
Ruyu Tang (Chongqing Normal University, Chongqing Engineering Research Center of Specialty Crop Resource, Chongqing,401331, China)
Jing Xiang (Chongqing Normal University, Chongqing Engineering Research Center of Specialty Crop Resource, Chongqing,401331, China)
Zhengwu Zhao (Chongqing Normal University, Chongqing Engineering Research Center of Specialty Crop Resource, Chongqing,401331, China)

Abstract


The genetic diversity of 36 rice landraces and 43 breeding materials in the upper reaches of the Yangtze River in China was studied by intragenic molecular markers of 26 starch synthesis-related loci. And research on quality traits such as the amylose content (AC), gel consistency (GC) and alkali spreading value (ASV) to analyze genetic differences in quality traits. The results showed that the number of alleles, average gene diversity and polymorphism information content values of landraces were higher than those of breeding materials. The genetic similarity coefficient (GS) of 79 rice materials ranged from 0.392 to 1, with an average of 0.757.There were significant variations in the quality traits of rice landraces and breeding materials, and the high-quality compliance rates were low, only 6.3% of the varieties have an amylose content that reached grade 1. The results of cluster analysis and population structure analysis are generally consistent; that is, the two resource types are closely related and cannot be clustered independently. This study can provide a basis for genetic improvement of rice starch quality. Make full use of the quality genetic diversity of landraces in modern breeding work, further broaden the genetic base of rice and improve rice quality.


Keywords


Genetic Diversity;Landrance;Population Structure;Rice;Starch Synthesis Related Genes

Full Text:

PDF

References


Liu CC, Zhao FW, Wu XX, Zhang CQ, Zhu ZK, Xue DY, Wu JY, Huang SW, Xu XY, Jin YG, Liu QQ. Genetic diversity and structure analysis of currently cultivated rice landraces from Hani’s terraced fields in Yunnan province. Chinese J Rice Science, 2015, 29(01): 28-34.

Wang DY, Zhang XF, Zhu ZW, Chen N, Min J, Yao Q, Yan JL, Liao XY.. Correlation Analysis of Rice Grain Quality Characteristics. Acta Agronomica Sinica, 2005, 31(08): 1086-1091.

Wang YZ, Wang XJ, Li Y, Xu H, Wang JY, Zhao MH, Tang L, Ma DR, Xu ZJ, Chen WF. Analysis of Yield and Quality Traits and Their Relationship in Japonica Rice in Northern China. Acta Agronomica Sinica, 2015, 41(6): 910-918.

Zeng YW, Zhang HL, Li ZC, Shen SQ, Sun JL, Wang MX, Liao DQ, Liu X, Wang XK, Xiao FH, Wen GS. Evolution of genetic diversity of rice landrance (Oryza sativa L.) in Yunnan, China. Breed Science, 2007, 57: 91-99.

Zhao WG, Chung JW, Ma KH, Kim TS, Kim SM, Shin DI, Kim CH, Koo HM, Park YJ. Analysis of Genetic Diversity and Population Structure of Rice Cultivars from Korea, China and Japan using SSR Markers. Genes Genom, 2009, 31(4): 283-292.

Zhu CL, Zhai HQ, Wan JM. Progresses in the Studies of Genetic and Molecular Bases of Eating- quality in rice. Acta Agriculturae Universitatis Jiangxiensis (Natural Sciences Edition), 2016, 24(04): 454-459.

Sun JC, Cao GL, Li YF, Ma J, Chen YF, Han LZ. Analysis of genetic diversity within populations of rice (Oryza sativa L.) Landrances. Journal of Northwest A & F University (Natural Sciences Edition), 2011, 39(12): 145-152.

Dai LY, Lin XH, Ye CR, Ise K, Saito K, Akira K, Xu FR, Yu TQ, Zhang DP. Identification of quantitative trait loci controlling cold tolerance at the reproductive stage in Yunnan landrace of rice Kunmingxiaobaigu. Breed Science, 2004, 54: 253-258.

Thomson MJ, Septiningsih EM, Suwardjo F, Santoso TJ, Silitonga, TS, Mccouch, SR. Genetic diversity analysis of traditional and improved Indonesian rice (Oryza sativa L.) germplasm using microsatellite markers. Theoretical and Applied Genetics, 2007, 114(3): 559-68.

Zhang XL, Guo H, Wang HG, Lv JZ, Yuan XP, Peng ST, Wei XH. Comparative Assessment of SSR Allelic Diversity in Wild and Cultivated Rice in China. Acta Agronomica Sinica, 2008, 34(4): 591-597.

Li HY, Hou YM, Chen YH, Xu ZJ, Chen WF, Zhao MH, Ma DR, Xu H, Wang JY. Evaluation on genetic diversity of the commercial rice varieties in northeast China by microsatellite markers. Chinese J Rice Science, 2009, 23(04): 383-390.

Cui XF. Study on the genetic diversity of starch synthesis related genes and association analysis of some starch quality traits with SSR markers of rice landrances in Tai Lake Region [master’s thesis]. Yangzhou(CHN): University of Yangzhou, 2011.

Murray MG, Thompson WF. Rapid isolation of high molecular weight plant DNA. Nucleic acids research, 1980, 8(19): 4321-4325.

Tian ZX, Yan CJ, Qian Q, Yan S, Xie HL, Wang F, Xu JF, Liu FG, Wang YH, Liu QQ, Tang SZ, Li JY, Gu MH. Development of gene-tagged molecular markers for starch synthesis-related genes in rice. Chinese Science Bulletin, 2010, 55(26): 2591-2601.

Liu KJ, Muse SV. PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics(Oxford, England), 2005, 21(9): 2128-2129.

Rohlf FJ. NTSYS-pc.Numerical taxonomy and multivariate analysis system, Version 2. 1. New York(NY): Exeter Software, 2000.

Evanno GS, Regnaut SJ, Goudet J. Detecting the number of clusters of individuals using the software STRUCTURE: A simulation study. Molecular Ecology, 2005, 14(8): 2611-2620.

Deng HZ, Wang CH, Xu Q, Yuan XP, Feng Y, Yu HY, Wang YP, Wei XH. Comparative Analysis of Genetic Diversity in Landrace and Improved Rice Varieties in China. Journal of Plant Genetic Resources, 2015, 16(3): 433-442.

Hua L, Yuan XP, Yu HY, Wang YP, Xu qun, Tang SX, Wei XH. A comparative study on SSR diversity in Chinese major rice varieties planted in 1950s and during the most recent ten years. Chinese J Rice Science, 2007, 21(02): 150-154.

Sun CQ, Wang XK, Li ZC, Yoshimura A, Iwata N. Comparison of the genetic diversity of common wild rice (Oryza rufipogon Griff.) and cultivated rice (O. sativa L.) using RFLP markers. Theoretical and Applied Genetics, 2001, 102(1): 157-162.

Qiang XT, Zhao CF, Zhao L, Zhao QY, Chen T, Zhou HL, Yao S, Wang CL. Analysis of genetic variation and population structure of starch synthesis related genes in indica rice cultivars. Jiangsu Journal of Agricultural Sciences, 2016, 32(2): 241-249.

Zhang SL,Yue HL,Zhao CF,Chen T,Zhang YD,Zhou LH,Zhao L,Liang WH,Wang CL. Allelic Variation of Starch Synthesis R elated Genes and Population Structure in Japonica Rice Cultivars. Acta Agriculturae Boreali-Sinica, 2018, 33(6): 116- 122.

Qi YW, Zhang DL, Zhang HL, Wang MX, Sun JL, Wei XH, Qiu ZG, Tang SH, Cao YS, Wang XK. Genetic diversity of rice cultivars ( Oryza sativa L.) in China and the temporal trends in recent fifty years. Chinese Science Bulletin, 2006, 51(6): 681-688.

Jin WD, Cheng BS, Hong DL. Genntic Diversity Analysis of Japonica Rice Landraces (Oryza sativa L.) in Tai Lake Region Based on SSR Markers. Scientia Agriculture Sinica, 2008, 41(11): 3822-3830.

Chen YH, Li HY, Hou YM, Xu ZJ, Yuan Y, Li MB. Genetic Diversity of Rice Germplasm Resources in Northeast Region of China. Acta Agriculturae Boreali-Sinica, 2009, 24(3): 165-173.

Lv GL, Lin ZL, Bai XG, Ma KH, Fu J, Liu FF, Huang XQ, Gway GG, Cheng ZQ. Comparative Assessment of Simple Sequence Repeat Genetic Diversity in Cultivated Rice from Yunnan. Bulletin of botany, 2009, 44(4): 457-463.

Ao Y. A genetic diversity assessment of starch quality traits in rice landraces from the Taihu basin, China. Journal of Integrative Agriculture, 2016, 15(3): 493- 501.

Tang ZM, Yang J, Wang XY, Cai KF, Li XF. Temporal change in diversity of grain quality traits in major conventional rice varieties in Guangdong Province, China. Chinese J Rice Sciene, 2012, 26(06): 669- 676.

Ma J, Sun CJ, Wang XS, Yang SL. Genetic Diversity and Genetic Relationship of Inbred Japonica Rice Varieties in Ningxia. Acta Botanica Boreali-Occidentalia Sinica. 2011, 31(5): 929-934.



DOI: https://doi.org/10.30564/jrb.v2i4.2346

Refbacks

  • There are currently no refbacks.
Copyright © 2020 Linyu Tai, Lixia Lei, Lan Luo, Hang Shen, Jiao Chen, Ruyu Tang, Jing Xiang, Zhengwu Zhao Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.