Effect of metformin on lactate metabolism in normal hepatocytes under high glucose stress in vitro

Xinxin Zhou (The First Clinical college of Hainan Medical University, Haikou 570100)
Xinliang Liu (college of Traditional Chinese Medicine, Hainan Medical University,Haikou 570100,China)
Ling Zhang (The First Clinical college of Hainan Medical University, Haikou 570100)
Yongyan Chen (The Second Clinical College of Hainan Medical University,Haikou 570100,China The First Clinical college of Hainan Medical University, Haikou 570100)
Shaoliang Guo (The Second Clinical College of Hainan Medical University,Haikou 570100,China)
Saijia Li (The First Clinical college of Hainan Medical University, Haikou 570100)
ZhiLu ZhiLu Li (The First Clinical college of Hainan Medical University, Haikou 570100)

Abstract


Objective To study the effect of metformin on lactate metabolism in hepatocytes in vitro under high glucose stress. Method: in vitro LO2 cells, liver cells were randomly divided into blank control group, 25 tendency/L glucose solution, 27 tendency/L glucose solution, 29 tendency/L glucose solution, 31 tendency/L glucose solution, 33 tendency/L glucose solution, 35 tendency/L glucose solution treatment group, determine the optimal concentration of 31 tendency after L, use 30 tendency for L metformin solution, and then divided into blank control group, the optimal concentration of glucose solution, normal liver cells + + metformin solution normal liver cells, The optimal concentration of glucose solution normal liver cells + + metformin solution respectively in the 12 h, 24 h, 48 h on cell count plate to calculate the number of liver cells, and using lactic acid determination kit the optimal concentration of glucose solution + normal liver cells and normal liver cells + the optimal concentration of glucose solution + metformin solution respectively in the 12 h, 24 h, 48 h of cell cultures of lactic acid value. Results: There was no significant change in the lactic acid concentration but significant increase in the number of surviving hepatocytes in the high-glycemic control group compared with that in the high-glycemic control group without metformin. Conclusion: Metformin has no significant effect on lactic acid metabolism of hepatocytes under high glucose stress in vitro, and has a protective effect on hepatocytes under high glucose stress. Based on this, it is preliminarily believed that metformin is not the direct factor leading to diabetic lactic acidosis.


Keywords


metformin; Liver cells; Metformin associated lactic acidosis (MALA)

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DOI: https://doi.org/10.30564/jer.v3i1.2864

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