Study of Condition Indices in Goby, Parachaeturichthys Ocellatus (Day, 1873) from the Creeks of Mumbai

Authors

  • Bindu Panicker Maharashtra College of Arts, Science and Commerce, Mumbai, India

DOI:

https://doi.org/10.30564/jfsr.v3i1.3253

Abstract

Condition indices study like RNA content, DNA content, RNA: DNA, RNA: protein, RNA: lipid was carried out in goby, Parachaeturichthys ocellatus from the creeks of Mumbai to assess its nutritional status in different months. The study was carried out from June 2010 to September 2011.The range of RNA content in male was 72-185.6 µg/100 mg while in female was 82-145.46 µg/100 mg. RNA content was high during spawning months. The DNA content showed slight variations with range of 22.56- 39.31 µg/100 mg in males and 25.20-32.52 µg/100 mg in females. The range of ratio of RNA: DNA in males was 2.08-5.13 with an average of 3.74 while in female was 2.92-5.07 with an average of 3.99. The ratio above 2 indicates good condition. The RNA: protein showed an average of 0.0015 in males and 0.0017 in females while the average of RNA: lipid was 0.0176 in males and 0.0127 in females. RNA: protein and RNA: lipid showed the lowest values in post reproductive stages while it increased with the onset of reproductive cycles. The condition indices study showed that P. ocellatus was in good condition throughout the year and the creeks of Mumbai were suitable habitat for feeding and reproduction.

Keywords:

Condition indices, RNA, DNA, protein, lipid

References

[1] Bulow, F.J. (1970), RNA-DNA ratio as indicators of recent growth rates of a fish, J.Fish. Res. Board .Can., 27: 2343-2349.

[2] Carter, C.G., Seeto, G.S., Smart, A., Clarke, S. And Van Barneveld , R.J. (1998), Correlates of growth in farmed juvenile Southern blue fin tuna Thunnus maccoyi (Castelnan), Aquaculture 161:107-119.

[3] Buckely, L.J., Cladorone, E. and Ong, T.L., (1999), RNA-DNA ratio and other nucleic acid based indicators for growth and condition of marine fishes, Hydrobiologia., 401: 265-277.

[4] Rooker, J.R and Holt, G.J. (1996), Application of RNA: DNA ratios to evaluate the condition and growth of larval and juvenile red drum (Sciaenops ocellatus), Mar. Freshwater Res. 47: 283-290.

[5] Vrede, T., Persson, J. and Aronsen, G. (2002), The influence of food quality (P:C ratio) on RNA: DNA ratio and somatic growth rate of Daphnia Limnol., Oceanogr. 47: 487-494.

[6] Clemmensen, C. (1994), The effect of food availability, age or size on the RNA/DNA ratio of individually measured herring larvae: laboratory calibration, Mar. Biol., 118: 377-382.

[7] Brown, M.L. and Austin, D.J. (1996), Data management and statistical techniques. In: B.R. Murphy. D.W. Willis (eds). Fisheries Techniques, American Fisheries Society, Bethesda, Maryland, pp 17-61.

[8] Gil, H., Chung, K.S., Lemus, M. and Altuve, D. (2003), RNA/DNA ratio as an index of physiological condition of Colossoma macropomum and Piaractus branchypomus (Pisces: Characiformes) during embryonic development, Rev. Biol. Trop 51.Supl 4 (2003): 91-96.

[9] Buckley, L.J. and Bulow, F.J. (1987), Techniques for the estimation of RNA,DNA and protein in fish. In age and Growth of fish (eds.) summerfelt and Hall, Iowa State University Press Ames, IOWA.

[10] Schneider, W.C. (1957), Determination of nucleic acids in tissues by pentose analysis. In Colowick, S.P and N.O Kalpan eds: Methods in Enzymology Vol.3 Academic Press, New York, pp1154.

[11] Webb, J.M. and Levy, H.B. (1955), A sensitive method for the determination of deoxyribonucleic acid in tissues and microorganisms, J. Biol. Chem, 213: 107- 117.

[12] Ashwell, G. (1957), Colorimeteric analysis of sugars: cysteine reaction of DNA In Colowick, S.P and N.O>kalpan eds: methods in enzymology Vol.3 Academic Press, New York, pp.1154.

[13] Lowry, O. H., Roserrough, N. J., Farr, A.L. and Randall, R.J. (1951), Protein Measurements with the Folin Phenol Reagent, J. Biol. Chem., 193, 265-276.

[14] Folch, J., Less, M. and Slone, S. (1957), A simple method for the isolation and purification of total lipids from animal tissues, J.Biol. Chem., 266: 497-509.

[15] Regnault, M. and Luquet, P. (1974), Study by evolution of nucleic acid content of prepuberal growth in the shrimp, Crangon vulgaris., Mar. Biol., 25: 291- 298.

[16] Chicharo, A.A., Pedro, M. and Luic, C. (2007), Effect of sex on ratios and concentration of DNA and RNA in three marine species, Marine Ecology Progress Sereis Vol. 332: 241-245.

[17] Chicharo, M.A. and Chicharo, L. (2008), RNA:DNA ratio and other nucleic acid derived indices in marine ecology, Intl. J.Mol.Sci., 9, 1453-1471.

[18] Paon, L.A. and Kenchington, E.L.R. (1995), Changes in somatic and reproductive tissues during artificial conditioning of sea scallop, Placopecten magellanicus (Gmelin, 1791), J. Shell. Fish Res., 14: 53-58.

[19] Perez Camacho, A., Delgado, M., Fernandez, R.M.J. and Labarta, U. (2003), Energy balance, gonad deveolpemt and biochemical composition in the clam Ruditapes decussates, Mar. Ecol. Prog. Ser., 258: 133- 145.

[20] Johannsson, O.E., Bowen, K.L., Arts, M.T., and Smith, R.W. (2009), Field assessment of condition indices (nucleic acid and protein) in Mysis diliviana,aquat.Biol.5:249-262.

[21] Zaboukas, N., Milious, H., Megalofonou, P and Moraitou- Apostolpoulou, M (2006), Biochemical composition of the Atlantic bonito Sarda sarda from the Aegean Sea (eastern Mediterranean Sea) in different stages of sexual maturity, Journal of Fish Biology (2006) 69, 347-362.

[22] Mustafa, S. and Zofair, S.M. (1985), Sesonal variations in Protein, RNA and DNA concentrations in major carps, Catla catla, Labeo rohita and Cirrhina mrigala, Japanese Journal of Ichthyology, Vol 32 (2) 258-262.

[23] Panicker, B.A 2021 Study of some aspects of reproductive biology of goby, Paracheaturichthys ocellatus (Day 1873) from the creeks of Mumbai, Int. J. Adv. Res. Biol. Sci. (2021). 8(3): 8-22.

[24] Foster, G.D., Houlihan, D.F. and Hall, H.J. (1993), Effects of nutritional regime on correlates of growth rate in juvenile Atlantic cod (Gadus morhua) comparison of morphological and biochemical measurements, Can. J. Fish. Aquat. Sci., 50: 502-512.

[25] Houlihan, D.F., Pannevis, M.C. and Heba, H. (1993), Protein synthesis in juvenile Tilapia Oreochromis mossambicus, J.World. Aquac. Soc., 24, 145-151.

[26] Smith, R.W., Palmer, P.M. and Houlihan, D.F. (2000), RNA turn over and protein synthesis in fish cells, J.Comp. Physiol., (B) 170: 135-144.

[27] Takama, K., Love, R.M. and Smith, G.L. (1985), Selectivity in mobilisation of stored fatty scids by maturing cod Gadus morhua, L, Comp. Biochem. Physiol. SOB 713-718.

Downloads

Issue

Vol. 3 , Iss. 1 (March 2021)

Article Type

Articles

License

Copyright and Licensing

The authors shall retain the copyright of their work but allow the Publisher to publish, copy, distribute, and convey the work.

Journal of Fisheries Science publishes accepted manuscripts under Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). Authors who submit their papers for publication by Journal of Fisheries Science agree to have the CC BY-NC 4.0 license applied to their work, and that anyone is allowed to reuse the article or part of it free of charge for non-commercial use. As long as you follow the license terms and original source is properly cited, anyone may copy, redistribute the material in any medium or format, remix, transform, and build upon the material.

License Policy for Reuse of Third-Party Materials

If a manuscript submitted to the journal contains the materials which are held in copyright by a third-party, authors are responsible for obtaining permissions from the copyright holder to reuse or republish any previously published figures, illustrations, charts, tables, photographs, and text excerpts, etc. When submitting a manuscript, official written proof of permission must be provided and clearly stated in the cover letter.
The editorial office of the journal has the right to reject/retract articles that reuse third-party materials without permission.

Journal Policies on Data Sharing

We encourage authors to share articles published in our journal to other data platforms, but only if it is noted that it has been published in this journal.