HPLC Study on HPLC Characteristic Spectrum of Huoxiang (Herba Agastachis) Eliminating Summer-heat Soft Capsule

Jing Wang (Wuhan institute for drug and medical device control, Wuhan, Hubei, China)

Article ID: 2955

DOI: https://doi.org/10.30564/jim.v10i1.2955

Abstract


To establish HPLC characteristic spectrum of Huoxiang (Herba Agastachis) Eliminating Summer-heat Soft Capsule. Chromatographic separation was performed on Tech Mate C18-ST (5 um, 4.6×250 nm) column by Agilent 1260 high performance liquid chromatograph, eluted with acetonitrile and 0.05% phosphoric acid in a gradient elution at a flow rate of 1.0 mL∙min-1. The detection wavelength was set at 270 nm. Hesperidin was selected as the reference peak to calculate the RSD of the relative retention time. Precision, stability and reproducibility were investigated as well. 80 different samples from 5 manufacturers were included in this study. The HPLC characteristic spectrum of Huoxiang Eliminating Summer-heat Soft Capsule was constructed with 11 specific chromatographic peaks in 80 samples. The method is accurate, reliable and with good reproducibility, and could overall control the quality of Huoxiang Eliminating Summerheat Soft Capsule.


Keywords


HPLC;HPLC characteristic spectrum;Huoxiang eliminating summer-heat soft capsule

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References


[1] Chinese Pharmacopoeia. 2015 edition. (Ministry of Public Health of the People’s Republic of China,2015).

[2] Feng Q, Luo CH. Determination of Hesperidin in Huoxiang Qushu Soft Capsules. HeiLongJiang Medicine Journal. 2006, Vol.19. No.4.

[3] Su YY, Liu F. Study on Quality-controlling Standard of Huoxiang (Herba Agastachis) Eliminating Summer-heat Soft Capsule. Chinese Traditional Patent Medicine. 2015, Oct. Vol.27. No.10.

[4] Luo M, Wei G, et al. HPLC study on HPLC characteristic spectrum of Dendrobium crepidatum. Modern Chinese Medicine, Apr. 2014. Vol. 16. No. 4.

[5] Ma QZ, Ma QW. A study on HPLC of the characteristic spectrum of Danshen tablets. Capital Medicine,2013, Vol. 10.

[6] Du JH, Zhang H. Study on Quality-controlling Standard of Huoxiang (Herba Agastachis) Eliminating Summer-heat Soft Capsule. Chinese Journal of Modern Applied Pharmacy, 2007, Sep. Vol. 24. No.8.

[7] Yao LW, Liu Y, et al. Application of the fingerprint and characteristic fingerprint technology in traditional Chinese medicinal materials and Chinese patent medicine. Chinese Journal of New Drugs.2018,27(8).

[8] Ha J, Wang JJ, et al. Study of HPLC-DAD characteristic chromatogram of Pyrrosia leaf formula granules.Journal of Hebei University of Science and Technology. 2017, Feb. Vol.38, No.1.

[9] Zhuo MZ, Zong NG, et al. Study on chromatographic fingerprint of sarcandra glabra (Thunb.) by microwave-assisted extraction coupled to HPLC/DAD. Academic Journal of Xi’an Jiaotong University. 2010,Nov. Vol.22, No.4.

[10] Miao WJ, Hu Y, et al. Profiling and identification of chemical components of Shenshao Tablet and its absorbed components in rats by comprehensive HPLC/DAD/ESI-MSN analysis. Chinese Journal of Natural Medicines. 2018, 16(10): 791-800.

[11] Zheng GF. Study on Huoxiang Qushu Soft capsule by TLC. Strait Pharmaceutical Journal. 2012, Vol.24.No.9.

[12] Ohtake N, Nakai Y, Yamamoto M, et al. Separation and isolation methods for analysis of the active principles of Sho-saiko-to (SST) oriental medicine. Journal of Chromatography B. 2004, 812:135.

[13] Jigar M, Vipul P, Nayan K, Niranjan V. A versatile LC method for the simultaneous quantification of latanoprost, timolol and benzalkonium chloride and related substances in the presence of their degradation products in ophthalmic solution. Analytical Methods,2010, 2, 1737-1744.


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