Bioefficacy of Ethanolic Leaves Extract of Azadirachta indica Against Stored Product Insect Pest, Tribolium castaneum (Herbst.)

Umar Aliyu (Department of Biological Sciences, Bauchi State University Gadau, P.M.B 65, Gadau, Bauchi State, Nigeria)
Sa’idu Kamal (Department of Biological Sciences, Bauchi State University Gadau, P.M.B 65, Gadau, Bauchi State, Nigeria)
Bashir Muhammad Abubakar (Department of Biological Sciences, Bauchi State University Gadau, P.M.B 65, Gadau, Bauchi State, Nigeria)
Isma’il Hassan (Department of Biological Sciences, Bauchi State University Gadau, P.M.B 65, Gadau, Bauchi State, Nigeria)


Tribolium castaneum is a species of beetle in the family tenebrionidae, the darkling beetle. Conventional insecticides used for controling stored product pests are expensive and arguably associated with various severe adverse side effects hence the need to develop botanical pesticides that are effective as alternative. Though Azadrachta indica has been used for the control of so many insects, review of the literature show no scientifically investigated report of its effectiveness against T. castaneum. This study was therefore designed to evaluate bioinsecticidal activity of ethanolic leaves extract of Azadirachta indica against stored Tribolium castaneum. Different concentrations (60, 70, 80, 90, and 100%) of ethanolic leaves extract of A. indica was applied on the filter paper and were allowed to dry for a reasonable time period. Control was maintained by treating the filter paper with water only. For examining the percent mortality, 15 adults was taken in the Petri dishes embedded with Whitman’s filter paper, covered with lid and tightened with scotch tape on both sides. The A. indica extracts at different concentrations tested showed insecticidal activity against T. castaneum. Further, phytochemical screening results showed that the A. indica extract have phytochemicals associated with insecticidal activity. The study has established that the ethanoloc extract A. indica is effective in managing stored product pest.


Azadrachta indica;Tribolium castaneum;Tenebrionidae ;Phytochemical

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