Effects of Genetic Variation in the 20th Intron of Sansui Duck ATP2A2 Gene on Eggshell Quality

Junhan Mei (College of Animal Science, Guizhou University, Guiyang 550025, Guizhou, China;Key Laboratory of Genetics, Breeding and Reproduction of Plateau and Mountain Animals of the Ministry of Education, Guizhou University, Guiyang 550025, Guizhou, China;Guizhou Province Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang 550025, Guizhou, China)
Yiyu Zhang (College of Animal Science, Guizhou University, Guiyang 550025, Guizhou, China;Key Laboratory of Genetics, Breeding and Reproduction of Plateau and Mountain Animals of the Ministry of Education, Guizhou University, Guiyang 550025, Guizhou, China;Guizhou Province Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang 550025, Guizhou, China)
Minfang You (College of Animal Science, Guizhou University, Guiyang 550025, Guizhou, China;Key Laboratory of Genetics, Breeding and Reproduction of Plateau and Mountain Animals of the Ministry of Education, Guizhou University, Guiyang 550025, Guizhou, China;Guizhou Province Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang 550025, Guizhou, China)
Chaomei Liao (College of Animal Science, Guizhou University, Guiyang 550025, Guizhou, China;Key Laboratory of Genetics, Breeding and Reproduction of Plateau and Mountain Animals of the Ministry of Education, Guizhou University, Guiyang 550025, Guizhou, China;Guizhou Province Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang 550025, Guizhou, China)
Guanghui Tan (College of Animal Science, Guizhou University, Guiyang 550025, Guizhou, China;Key Laboratory of Genetics, Breeding and Reproduction of Plateau and Mountain Animals of the Ministry of Education, Guizhou University, Guiyang 550025, Guizhou, China;Guizhou Province Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang 550025, Guizhou, China)
Jiezhang Li (College of Animal Science, Guizhou University, Guiyang 550025, Guizhou, China;Key Laboratory of Genetics, Breeding and Reproduction of Plateau and Mountain Animals of the Ministry of Education, Guizhou University, Guiyang 550025, Guizhou, China;Guizhou Province Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang 550025, Guizhou, China)

Abstract


In order to explore the influence of the polymorphism in the 20intron region of the Sansui duck ATP2A2 gene on the eggshell quality, this study used Primer Premier 5 software to design and synthesize a pair of primers in the 20 intron region, two-way direct sequencing and sequence alignment to mine SNPs Sites, SPSS 18.0 software was used to analyze the relationship between SNP sites and eggshell quality of Sansui duck.Three SNP sites were found in the 20 intron region of the ATP2A2 gene: g.40874 T>C, g.40920 G>A and g.40990 T=C, all of which were moderately polymorphic, at the site g.40874 T>C and g.40920 G>A both deviated significantly from Hardy-Weinberg equilibrium (P>0.05), position g.40990 T=C accords with Hardy-Weinberg equilibrium (P<0.05), and position g.40874 T>C There is a strong linkage disequilibrium between g.40990 T=C; a total of 4 haplotypes and 9 double types were detected at 3 SNP loci; the results of association analysis showed that g.40874 T>C mutation had a significant effect on eggshell strength and eggshell weight.The eggshell strength of CC genotype was significantly higher than that of TC and TT genotypes (P<0.05), the eggshell weight of CC genotype was significantly higher than that of TC genotype (P<0.05), and 40990 T=C mutation had a significant effect on eggshell strength. The eggshell strength of TC genotype was significantly higher than that of the TT genotype (P<0.05). In summary, the g.40874 T>C and g.40990 T=C found in the 20th intron region of the Sansui duck ATP2A2 gene may be the marker sites that affect the quality of the eggshell.


Keywords


Sansui duck;ATP2A2 gene;SNP locus;Eggshell quality

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References


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DOI: https://doi.org/10.30564/jzr.v3i2.3048

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Copyright © 2021 Junhan Mei, Yiyu Zhang, Minfang You, Chaomei Liao, Guanghui Tan, Jiezhang Li


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