Effects of Genetic Variation in the 20th Intron of Sansui Duck ATP2A2 Gene on Eggshell Quality

Authors

  • Junhan Mei College of Animal Science, Guizhou University, Guiyang 550025, Guizhou, China;Key Laboratory of Genetics, Breeding and Reproduction of Plateau and Mountain Animals of the Ministry of Education, Guizhou University, Guiyang 550025, Guizhou, China;Guizhou Province Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang 550025, Guizhou, China
  • Yiyu Zhang College of Animal Science, Guizhou University, Guiyang 550025, Guizhou, China;Key Laboratory of Genetics, Breeding and Reproduction of Plateau and Mountain Animals of the Ministry of Education, Guizhou University, Guiyang 550025, Guizhou, China;Guizhou Province Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang 550025, Guizhou, China
  • Minfang You College of Animal Science, Guizhou University, Guiyang 550025, Guizhou, China;Key Laboratory of Genetics, Breeding and Reproduction of Plateau and Mountain Animals of the Ministry of Education, Guizhou University, Guiyang 550025, Guizhou, China;Guizhou Province Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang 550025, Guizhou, China
  • Chaomei Liao College of Animal Science, Guizhou University, Guiyang 550025, Guizhou, China;Key Laboratory of Genetics, Breeding and Reproduction of Plateau and Mountain Animals of the Ministry of Education, Guizhou University, Guiyang 550025, Guizhou, China;Guizhou Province Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang 550025, Guizhou, China
  • Guanghui Tan College of Animal Science, Guizhou University, Guiyang 550025, Guizhou, China;Key Laboratory of Genetics, Breeding and Reproduction of Plateau and Mountain Animals of the Ministry of Education, Guizhou University, Guiyang 550025, Guizhou, China;Guizhou Province Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang 550025, Guizhou, China
  • Jiezhang Li College of Animal Science, Guizhou University, Guiyang 550025, Guizhou, China;Key Laboratory of Genetics, Breeding and Reproduction of Plateau and Mountain Animals of the Ministry of Education, Guizhou University, Guiyang 550025, Guizhou, China;Guizhou Province Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang 550025, Guizhou, China

DOI:

https://doi.org/10.30564/jzr.v3i2.3048

Abstract

In order to explore the influence of the polymorphism in the 20intron region of the Sansui duck ATP2A2 gene on the eggshell quality, this study used Primer Premier 5 software to design and synthesize a pair of primers in the 20 intron region, two-way direct sequencing and sequence alignment to mine SNPs Sites, SPSS 18.0 software was used to analyze the relationship between SNP sites and eggshell quality of Sansui duck.Three SNP sites were found in the 20 intron region of the ATP2A2 gene: g.40874 T>C, g.40920 G>A and g.40990 T=C, all of which were moderately polymorphic, at the site g.40874 T>C and g.40920 G>A both deviated significantly from Hardy-Weinberg equilibrium (P>0.05), position g.40990 T=C accords with Hardy-Weinberg equilibrium (P<0.05), and position g.40874 T>C There is a strong linkage disequilibrium between g.40990 T=C; a total of 4 haplotypes and 9 double types were detected at 3 SNP loci; the results of association analysis showed that g.40874 T>C mutation had a significant effect on eggshell strength and eggshell weight.The eggshell strength of CC genotype was significantly higher than that of TC and TT genotypes (P<0.05), the eggshell weight of CC genotype was significantly higher than that of TC genotype (P<0.05), and 40990 T=C mutation had a significant effect on eggshell strength. The eggshell strength of TC genotype was significantly higher than that of the TT genotype (P<0.05). In summary, the g.40874 T>C and g.40990 T=C found in the 20th intron region of the Sansui duck ATP2A2 gene may be the marker sites that affect the quality of the eggshell.

Keywords:

Sansui duck, ATP2A2 gene, SNP locus, Eggshell quality

References

[1] Li C, Xiao Z, Peng Y, et al. A novel mutation in exon 1 of the ATP2A2 gene in four members of one Chinese family with Darier disease. Clinical and Experimental Dermatology, 2020, 45(7):925-928.

[2] Xia Qianqian, Zhao Qing, Sun Lele, et al. Study on ATP2A2 gene mutation in a family of keratosis follicularis and three sporadic patients[J].China Journal of Leprosy and Skin Diseases, 2019, 35(07):385-388.

[3] Zhao Q, Fu FH, Fu XA, et al. Three novel mutations of the ATP2A2 gene in Chinese patients with Darier disease. 2019, 60(2): e171-e172.

[4] Chen Siyuan, Huang Changzheng, Qian Yue, et al.Detection of ATP2A2 gene mutation in a family of follicular keratosis[J]. China Journal of Leprosy and Skin Diseases, 2006(07): 549-551.

[5] Ardlie KG, Kruglyak L, Seielstad M. Patterns of linkage disequilibrium in the human genome[J].Nature Reviews Genetics, 2002, 3(4): 299-309.

[6] Slatkin M. Linkage disequilibrium-understanding the evolutionary past and mapping the medical future[J]. Nature Reviews Genetics, 2008, 9(6): 477-485.

[7] Mohamed K, Eva T, Michaela E, et al. Combined Effect of Genotype, Housing System, and Calcium on Performance and Eggshell Quality of Laying Hens.Animals: an open access journal from MDPI, 2020,10(11): 2120.

[8] Yang Yang, Ma Lin, Liu Qiuyue, et al. Association analysis between LHCGR gene polymorphism and litter size in sheep[J]. Chinese Herbivore Science,2019, 39(06): 1-6+21.

[9] Mayo O. A century of Hardy-Weinberg equilibrium[J]. Twin Research and Human Genetics, 2008,11(3): 249-256.

[10] Ren G, Huang YZ, Wei TB, et al. Linkage disequilibrium and haplotype distribution of the bovine LHX4 gene in relation to growth[J]. Gene, 2014, 538(2):354-360.

[11] Jiang Danli, Zhang Zhuoshen, Sun Junfeng, et al.The effect of a small amount of eggshell powder in place of stone powder on laying performance, egg quality and calcium metabolism of laying ducks[J].Guangdong Agricultural Sciences, 2020, 47(03): 117-122.

[12] Tan Guanghui, Li Jiezhang, Qin Yuanyu, et al. Correlation between genetic variation of Sansui duck ORAI1 gene and eggshell quality[J]. Journal of Fujian Agriculture and Forestry University (Natural Science Edition), 2020, 49(05): 646-651.

[13] Luo Yiqiu, Kuang Wei, He Zongliang, et al. Study on the Variation Law of Egg Quality of Jinling White Duck at Different Laying Weeks[J]. Journal of Anhui Agricultural Sciences, 2020, 48(22):107-109+147.

[14] Li Jiezhang, Tan Guanghui, Wu Lei, et al. Identification of SNPs in ITPR1 gene of Sansui duck and its association with eggshell quality traits[J]. Journal of Agricultural Biotechnology, 2020, 28(03):455-464.

Downloads

Issue

Vol. 3 , Iss. 2 (April 2021)

Article Type

Reviews

License

Copyright and Licensing

The authors shall retain the copyright of their work but allow the Publisher to publish, copy, distribute, and convey the work.

Journal of Zoological Research publishes accepted manuscripts under Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0). Authors who submit their papers for publication by Journal of Zoological Research agree to have the CC BY-NC 4.0 license applied to their work, and that anyone is allowed to reuse the article or part of it free of charge for non-commercial use. As long as you follow the license terms and original source is properly cited, anyone may copy, redistribute the material in any medium or format, remix, transform, and build upon the material.

License Policy for Reuse of Third-Party Materials

If a manuscript submitted to the journal contains the materials which are held in copyright by a third-party, authors are responsible for obtaining permissions from the copyright holder to reuse or republish any previously published figures, illustrations, charts, tables, photographs, and text excerpts, etc. When submitting a manuscript, official written proof of permission must be provided and clearly stated in the cover letter.
The editorial office of the journal has the right to reject/retract articles that reuse third-party materials without permission.

Journal Policies on Data Sharing

We encourage authors to share articles published in our journal to other data platforms, but only if it is noted that it has been published in this journal.