A Simple Procedure for Extraction of Surface Protein of Salmonella Serotypes and Escherichia coli Strains Isolated from Poultry and Pigs

Authors

  • Tran Thi Quynh Lan Department of Veterinary Biosciences, Faculty of Animal Science and Veterinary Medicine Nong Lam University, Ho Chi Minh City
  • Doan Thi Da Linh Faculty of Animal Science and Veterinary Medicine, Nong Lam University, Ho Chi Minh City
  • Le Ho Truc Phuong Faculty of Animal Science and Veterinary Medicine, Nong Lam University, Ho Chi Minh City

DOI:

https://doi.org/10.30564/vsr.v1i2.1357

Abstract

Salmonella and E.coli possess different surface protein structures that can induce protective immune responses. Identification of these proteins capacitates development of diverse applications in prevention and diagnosis that contribute to effectively control disease-causing enterobacteria pathogens such as Salmonella and E.coli. A simple procedure for obtaining protein complexes of Salmonella serotypes and E.coli is performed in this study. A sonication process with heat treatment of whole bacteria induced the release of protein complexes. Concentration of the protein extract was quantified using protein quantification Kits-Rapid, and protein complex profile was obtained by SDS-PAGE (Sodium dodecyl sulfate polyacrylamide gel electrophoresis) and silver staining. The concentrations of protein ranged from 29.45 to 45.35 µg/mL in the  Salmonella protein extracts, and from 25.35 to 36.72 µg/mL in the E.coli protein extracts. Six major groups of proteins from E. coli (YfiO, NipB, OmpF, YfgL, Talc, YaeT) and four major groups of proteins from Salmonella (Flagellin, OmpA, Porin, SEF21) were preliminarily determined by a simple procedure of extraction based on the molecular weight.

Keywords:

Salmonella serotypes, E.coli strains, Extraction, Protein complexes extract, SDS-PAGE

References

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Vol. 1 , Iss. 2 (November 2019)

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