Multiple RT-PCR Detection of H5, H7, and H9 Subtype Avian Influenza Viruses and Newcastle Disease Virus

Feng Fei (Jiaxing Vocational & Technical College, Jiaxing, Zhejiang, 314036, China)

Abstract


Objective: This paper focuses on the multiple detection RT-PCR technology of H5, H7, AND H9 subtype avian influenza viruses and Newcastle disease virus, and points out the specific detection methods and detection procedures of avian influenza and Newcastle disease virus. Methods: The genes of Newcastle disease virus carrying out the HA gene sequence of H5, H7 and H9 subtype AIV in GenBank were used to establish a strategy for simultaneous detection of three subtypes of avian influenza virus and Newcastle disease virus. Results: The results showed that the program can detect and distinguish H5, H7 and H9 subtype avian influenza viruses and Newcastle disease virus at one time. Conclusion: Multiple RT-PCR detection method has high detection sensitivity and can detect and determine different subtypes of avian influenza virus and Newcastle disease virus quickly and accurately, therefore, it has a crucial role in the detection and control of avian influenza H5, H7 and H9 subtypes and Newcastle disease.


Keywords


H5, H7 and H9 subtype avian influenza viruses; Newcastle disease virus (NDV); RT-PCR

Full Text:

PDF

References


[1] References

[2] Menghou Gan. Avian Influenza[M]. Beijing: China Agricultural Press, 2002. (in Chinese)

[3] Hualan Chen, Kangzhen Yu, Guobin Tian. RTPCR amplification and cloning of HA gene of H5 and H7 subtype avian influenza viruses[J].Chinese Journal of Animal and Infectious Diseases,1997,19(2):16-18. (in Chinese)

[4] Xiumei Zhang, Khan MI. Diagnosis of mixed infection of avian Mycoplasma and other respiratory pathogens by composite RT-PCR[J].China Veterinary Science & Technology,2003,33(3):232-234. (in Chinese)

[5] Yaoshan Pang, Zhixun Xie, Khan MI. Establishment of a method for simultaneous detection of six respiratory diseases in chickens by two-temperature multiple PCR [J]. Chinese Veterinary Science and Technology, 2001, 31(5):3-6. (in Chinese)

[6] Xiaojun Peng, Guobao Wang, Yan Wang, et al. Diagnosis of H9 subtype avian influenza virus and Escherichia coli infection[J]. Chinese Journal of Health Inspection, 2018, 28(19):46-48. (in Chinese)

[7] Jie Zheng, Yubang He. Thoughts on the prevention and control of wild bird avian influenza in Qinghai Lake area[J]. Qinghai Science and Technology, 2006(1):8-10. (in Chinese)

[8] Xinyou Yu, Tianzhi Li, Jinliang Wang, et al. Establishment of a one-step multiple RT-PCR method for detection of Newcastle disease virus, H9 subtype avian influenza virus and infectious bursal disease virus And application[J]. Poultry and Poultry Disease Prevention, 2016(4):8-13. (in Chinese)

[9] Zhiqin Xie, Zhixun Xie, Qing Fan, et al. Establishment of double RT-PCR detection method for H9 subtype avian influenza virus and avian lung virus[J]. Advances in Animal Medicine, 2016, 37(12):7-12. (in Chinese)



DOI: https://doi.org/10.30564/vsr.v1i2.1429

Refbacks

  • There are currently no refbacks.
Copyright © 2020 feng fei


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.