Epidemiology and Antibiotic Succeptibility Profile of Methicillin Sensitive Staphylococcus aureus among Livestock and Pet Animals

Muhammad Aamir Naseer (Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad-38000, Pakistan)
Amjad Islam Aqib (Department of Medicine, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, 63100, Pakistan)
Muhammad Shoaib (Institute of Microbiology, University of Agriculture, Faisalabad, 38000, Pakistan)
Iqra Muzammil (Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad-38000, Pakistan)
Zeeshan Ahmad Bhutta (Royal (Dick) School of Veterinary Studies, The University of Edinburgh, United Kingdom)
Iqra Gulzar (Department of Zoology and Fisheries, University of Agriculture, Faisalabad-38000, Pakistan)

Abstract


Staphylococcus aureus is an important zoonotic pathogen that is responsible for a variety of infectious diseases in humans and animals. The present study was designed to check the prevalence and antimicrobial resistance of MSSA from three different animal origins (bovine, caprine and pet). A total of n= 450 samples (150 each source) were collected from bovine, caprine and pets. Collected samples were subjected to S. aurues identification by microbiological examination and confirmed S. aurues isolates were put to oxacillin disk diffusion test to declare them MSSA. The MSSA confirmed isolates were subjected to various antibiotics for susceptibility profiling using Kirby Baur Disk Diffusion test. The present study found higher prevalence of MSSA from caprine origin (goat 83.33%) as compared to pet (cat 69.33%; dog 65.33%) and bovine origin (buffalo 26.66%; cattle 31.66%). The in-vitro findings of current study revealed oxytetracycline and gentamicin presented 100% efficacy against MSSA of all origins while the vancomycin presented >35%, >40% and > 65% resistance against MSSA isolated from bovine, caprine and pet origin respectively. However, ciprofloxacin was equally effective (50%) against MSSA from buffalo and cattle while >80% efficacy was noted against MSSA from cat and dogs. Linezolid and amoxicillin+ clavulanic acid were 77.78% and 66.67% sensitive to MSSA isolates from caprine milk. The present study found higher prevalence of MSSA from bovine, caprine and pet isolates with diversified pattern of susceptibility of different antibiotics from all sources.


Keywords


S. aureus; MSSA; Pet; Bovine; Caprine; Antibiotic susceptibility

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References


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DOI: https://doi.org/10.30564/vsr.v2i1.1902

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Copyright © 2020 Iqra Muzammil, Muhammad Shoaib, Amjad Islam Aqib, Muhammad Aamir Naseer, Zeeshan Ahmad Bhutta


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