Protective Properties in Hymenaea martiana Hayne against Multi-drug-resistant Staphylococcus aureus

Dielson da Silva Vieira (São Paulo State University “Júlio de Mesquita Filho” (UNESP), School of Veterinary Medicine, Araçatuba, Sao Paulo, Brazil)
Tânia Maria Sarmento da Silva (Federal Rural University of Pernambuco (UFRPE), Department of Molecular Sciences, Recife, Pernambuco, Brazil)
Timothy A. Hackett (Department of Biochemistry, University of Nebraska - Lincoln, Lincoln, NE, 68508, USA)
Mariana de Barros (Federal University of Viçosa (UFV), Veterinary Department, Viçosa, Minas Gerais, Brazil)
Weslen Fabrício Pires Teixeira (Federal University of Goiás (UFG), Department of Veterinary Medicine, Goiânia, Goiás, Brazil)
Juliana Campos Pereira Diniz (São Paulo State University “Júlio de Mesquita Filho” (UNESP), School of Veterinary Medicine, Araçatuba, Sao Paulo, Brazil)
Pedro Henrique Gorni (University of West Paulista (UNOESTE), Department of Agronomy, Presidente Prudente, Sao Paulo, Brazil)
Sanely Lourenço da Costa (Federal University of Viçosa (UFV), Veterinary Department, Viçosa, Minas Gerais, Brazil)
Maria Aparecida Scatamburlo Moreira (Federal University of Viçosa (UFV), Veterinary Department, Viçosa, Minas Gerais, Brazil)
Mateus Matiuzzi da Costa (Federal University of Vale do São Francisco (UNIVASF), Department of Animal Science, Petrolina, Pernambuco,Brazil)
Francisco Leydson Formiga Feitosa (São Paulo State University “Júlio de Mesquita Filho” (UNESP), School of Veterinary Medicine, Araçatuba, Sao Paulo, Brazil)

Abstract


Antibiotic resistance represents a widespread problem in milk production. The identification of compounds for a topically applied ointment used in mastitis therapy remains elusive. Compounds from the genus Hymenaea can be administered in cases of multi-drug-resistant Staphylococcus aureus infection for ruminant species, but the protective properties are not well known. Wi this research the aim is verify the protective effects of H. martiana against S. aureus infection in bovine mammary epithelial cell line (MAC-T) and to obtain an antioxidant profile evaluation in vitro. The MAC-T cells were challenged with S. aureus after being exposed to the extract of the H. martiana in the protective assay. For the verification of the viability of the MAC-T cells, the MTT assay was performed, and was used dilutions of the plant extract, starting at 2.5%. The extract of H. martiana was evaluated for antioxidant aspect in different dilutions by FRAP, ORAC and DPPH. A variety of flavonoids (quercetin, luteolin, etc.) have been identified as the main components by using mass spectrometry, reinforcing our in vitro findings that flavonoids, especially quercetin, have a medicinal profile capable of killing mastitis-causing bacteria. An excellent antioxidant pattern was observed in the 2.5% solution; however, membrane integrity in MAC-T cells was compromised. Those findings suggest low dilutions of H. martiana extract has a desirable protective effect from S. aureus pathogenesis. Our in vitro studies can be gleaned upon for further in vivo studies.


Keywords


Dairy animal; Fitoterapic; Hymenaea gender; Cell activity; Mastitis

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References


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DOI: https://doi.org/10.30564/vsr.v2i2.2528

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